鸽蛋低密度脂蛋白对冷休克和冻融猪精子HSP70及凋亡相关基因表达的影响

doi:10.11843/j.issn.0366-6964.2015.06.009

Abstract

Abstract:

The purpose of this study is to investigate the effects of LDL from pigeon egg yolk on expression of HSP70 and apoptosis related genes in cold-shocked and frozen-thawed boar spermatozoa.This study divided into 5 groups：fresh sperm group，3% LDL（-80 and -196 ℃） treatment groups，9%LDL（-80 and -196 ℃）treatment groups.The addition of LDL with different concentrations has effect on biological features of the spermatozoa before and after cold-shock and frozen-thawed treatments ，which was analysed by CASA，and changes in expression of HSP70 and apoptosis-related genes were analysed and checked by SDS-PAGE，Western blotting and PCR.The results showed that：HSP70 expression was higher than that of fresh sperm after freezing，there was no significant difference between HSP70 expression of frozen-thawed stored at -196 ℃ with addition of 9% pigeon egg and fresh spermatozoa in treatment group（P＞0.05）；in the experiment of anti-apoptosis gene Bcl-x1，Bcl-21，gene expression in fresh sperm treatment group was higher than other treatment groups（P＞0.05）；in the expression of pro-apoptotic Bak gene，the expression of fresh sperm was the lowest，there was no significant difference between Bak gene of the 9% LDL(-196 ℃) treatment group and fresh spermatozoa in treatment group（P＞0.05）.Addition of LDL from pigeon egg yolk could reduce the damage caused by freezing and thawing，maintain biological features and improve the survival of the pig spermatozoa.

CLC Number:

S813.3

LIU Yang，HUANG He-lu，DONG Hai-tao，JIN Yi. Effect of LDL from Pigeon Egg Yolk on the Expression of HSP70 and Apoptosis-related Gene in Cold Shock and Frozen-thawed Boar Spermatozoa[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 940-948.

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References

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Effect of LDL from Pigeon Egg Yolk on the Expression of HSP70 and Apoptosis-related Gene in Cold Shock and Frozen-thawed Boar Spermatozoa

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